响应面法优化地黄多糖铁(Ⅲ)合成工艺

刘冲英, 李瑾, 周宁, 崔涛, 张婷婷, 蒲定涛, 陈艳, 张丽华, 赵鹏

中国药学杂志 ›› 2020, Vol. 55 ›› Issue (20) : 1703-1710.

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中国药学杂志 ›› 2020, Vol. 55 ›› Issue (20) : 1703-1710. DOI: 10.11669/cpj.2020.20.008
论著

响应面法优化地黄多糖铁(Ⅲ)合成工艺

  • 刘冲英1, 李瑾1, 周宁2, 崔涛3, 张婷婷1, 蒲定涛1, 陈艳1, 张丽华1, 赵鹏1*
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Optimization of Synthesis of Rehmannia glutinosa Libosch Polysaccharide-Iron(Ⅲ) by Response Surface Method

  • LIU Chong-ying1, LI Jin1, ZHOU Ning2, CUI Tao3, ZHANG Ting-ting1, PU Ding-tao1, CHEN Yan1, ZHANG Li-hua1, ZHAO Peng1*
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文章历史 +

摘要

目的 以地黄多糖为原料,合成地黄多糖铁(Ⅲ)。方法 在单因素实验的基础之上,通过响应面分析法优化合成地黄多糖铁(Ⅲ)的最优工艺条件,利用红外光谱法(IR)和扫描电镜(SEM)对其结构进行表征,并通过模拟胃肠道环境测定其铁离子释放率。结果 合成地黄多糖铁(Ⅲ)的最佳工艺条件为:m(三氯化铁):m(地黄多糖)=2.0,m(枸橼酸三钠):m(地黄多糖)=1.45,80 ℃,pH 8.0,反应时间1.05 h,地黄多糖铁(Ⅲ)中铁含量可达16.03%。IR和SEM的实验结果证实,地黄多糖和铁(Ⅲ)发生了反应。经过体外溶出实验和还原性实验可知地黄多糖铁(Ⅲ)具有易溶易还原的特点,且在模拟胃肠道环境下,铁 (Ⅲ)在60 min内的释放率达80%以上。结论 地黄多糖铁(Ⅲ)具有含铁量高、溶解性好等优点,有望开发成一类新型补铁剂。

Abstract

OBJECTIVE To synthesize Rehmannia glutinosa Libosch polysaccharide-iron (Ⅲ) via polysaccharide from Rehmannia glutinosa Libosch. METHODS The synthetic process was optimized via response surface method on the basis of single factor experiment. Its chemical structure was characterized by infrared spectrum (IR) and scanning electron microscope (SEM). Its drug release percentage was determined in simulated gastrointestinal liquid. RESULTS The optimum conditions were as follows: m(ferric chloride): m(Rehmannia glutinosa Libosch polysaccharide)=2.0, m(trisodium citrate): m(Rehmannia glutinosa Libosch polysaccharide)=1.45, 80 ℃, pH 8.0, and reaction time of 1.05 h. Under above conditions, the iron content of Rehmannia glutinosa Libosch polysaccharide-iron (Ⅲ) reached 16.03%. The IR and SEM indicated that Rehmannia glutinosa Libosch polysaccharide was combined with iron (Ⅲ). Rehmannia glutinosa Libosch polysaccharide-iron (Ⅲ) could be dissolved and reduced easily as shown by in vitro dissolution experiment and reducing experiment, and the cumulative release percentage of iron (Ⅲ) was more than 80% within 60 min in stimulated gastrointestinal liquid. CONCLUSION Rehmannia glutinosa Libosch polysaccharide-iron (Ⅲ) has the advantages of high iron content and good solubility. It can be developed as a new iron supplementary.

关键词

地黄多糖铁(Ⅲ) / 合成 / 响应面法 / 优化

Key words

Rehmannia glutinosa Libosch polysaccharide-iron(Ⅲ) / synthesis / response surface method / optimization

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导出引用
刘冲英, 李瑾, 周宁, 崔涛, 张婷婷, 蒲定涛, 陈艳, 张丽华, 赵鹏. 响应面法优化地黄多糖铁(Ⅲ)合成工艺[J]. 中国药学杂志, 2020, 55(20): 1703-1710 https://doi.org/10.11669/cpj.2020.20.008
LIU Chong-ying, LI Jin, ZHOU Ning, CUI Tao, ZHANG Ting-ting, PU Ding-tao, CHEN Yan, ZHANG Li-hua, ZHAO Peng. Optimization of Synthesis of Rehmannia glutinosa Libosch Polysaccharide-Iron(Ⅲ) by Response Surface Method[J]. Chinese Pharmaceutical Journal, 2020, 55(20): 1703-1710 https://doi.org/10.11669/cpj.2020.20.008
中图分类号: TQ28   

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基金

国家重点研发计划项目资助(2018YFC1706903);国家自然科学基金项目资助(81403101);陕西省自然科学基金项目资助(2019JM-502);陕西省重点实验室基金项目资助(19JS019);陕西省重点研发计划项目资助(2020SF-315)
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